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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by <t>optic</t> <t>grid</t> fluorescence <t>microscopy</t> (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.
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Image Search Results


The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by optic grid fluorescence microscopy (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.

Journal: PLoS ONE

Article Title: The Effect of Gentamicin-Induced Readthrough on a Novel Premature Termination Codon of CD18 Leukocyte Adhesion Deficiency Patients

doi: 10.1371/journal.pone.0013659

Figure Lengend Snippet: The two patients' Epstein-Barr virus-transformed cells were treated with gentamicin (1000 µg/ml) for 5 days. Cells were cytospun, fixed and immunostained with mouse monoclonal anti integrin β2 (CD18) antibody (Santa Cruz Biotechnology Inc., USA). Slides were mounted using immunofluorescence and DAPI for nuclear staining and analyzed by optic grid fluorescence microscopy (Olympus). a. 1000-fold magnification of healthy control's Epstein-Barr virus-transformed lymphocytes b. 600-fold magnification of gentamicin untreated (upper panel) and treated (lower panel) lymphoblastoids derived from patients 1 and 2. c. 1000-fold magnification of gentamicin untreated (left panel) and treated (right panel) lymphoblastoids derived from patient 1. N indicates nucleus; PM, plasma membrane; C, cytoplasm.

Article Snippet: The slides were mounted using immunofluore containing 4′,6-diamidino-2-phenylindolem (DAPI) for nuclear staining, and analyzed by optic grid fluorescent microscopy (Olympus).

Techniques: Transformation Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Derivative Assay